SKU: 31826181479

Mouse TGAB (anti-Thyroid-Globulin Antibody) ELISA Kit

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Description

Mouse TGAB (anti-Thyroid-Globulin Antibody) ELISA KitThe Mouse TGAB (anti Thyroid Globulin Antibody) ELISA Kit measures TG Ab in Mouse Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antigen Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with antigen. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection

The Mouse TGAB (anti-Thyroid-Globulin Antibody) ELISA Kit measures TG-Ab in Mouse Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antigen-Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with antigen. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection antigen. Then, it binds with TGAB bound to precoated antigen. Wash unbound components and add HRP-Streptavidin Conjugate (SABC). Wash unbound components again and add TMB substrate solution. Then, TMB was catalyzed by HRP to produce a blue color product that turned yellow after adding a stop solution. Read the O.D. absorbance at 450nm in a microplate reader. Calculate the concentration of TGAB in the sample by plotting standard curve. The concentration of the target substance is proportional to the OD450 value.

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SKU: 31826181479

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